Laboratory of subtropical plants

  • Research on problems relevant to adaptation in the environment, growth and development, cultivation techniques and propagation of some tropical and subtropical fruit plants which appear to be economically important for Greece like: Avocado, Mango, Loquat, Papaya, Jojoba, Carob, Cherimoya, Date Palm, Pecan, Litchi, Persimmon, Cactus Pear, Pomegranate.
  • Study and evaluation of the rootstocks concerning their adaptation and resistance to different diseases.
  • Production and conservation of healthy genetic material of citrus.
  • Propagation of plants by applying either conventional techniques (seedlings, grafting, rooting cuttings) or biotechnological methods (in vitro culture of tissues or organs
  • Study of soil ecosystem and plant microbe interactions as well as their significance for sustainable agriculture
  • Surveys for CTV virus of citrus plants

 frouta2  frouta1  frouta3 frouta8 frouta5 frouta4  

  • Were evaluated the new late navel orange varieties "Navel Late" and "Lane Late" as well as the super early clone of navel orange "RO 25" that is a choice of Institute of Olive tree and Subtropical plants of Chania.
  • Varieties of 'Bergamot' (collection of Institute)
  • Evaluation of the recommended cultivars 'Marisol', 'Nova', 'Clasuelina' and 'Miyagava wase'. The cultivars 'Marisol' and 'Miyagava wase' reach maturity in October while the hybrid 'Nova' allows picking in November and sustains its outstanding characteristics very well, until February.
  • New rootstocks were studied and some of them were singled out for their good performance under the conditions of Crete. The rootstocks of Sour oranges (varieties Brazilian and Bittersweet) are tolerant to saline soils. The rootstock citrange 'Carrizo' is ideal for early varieties, and the citrumelo 'Swingle' for the late varieties.
  • At the installations of the Institute indexing for virus control was carried out. The disease 'exocortis' is the most common one. So that, for the reconstitution of citrus, the varieties to be used in future programs is necessary to use rootstocks tolerant to the viroid 'exocortis'.
  •  Certified propagative material of various tropical and subtropical fruit crops was imported, from research Institutes of other counties, and the possibility of their successful cultivation in Greece was studied. Evaluation and selection of the most important cultivars of Avocado, Kiwi, Banana, Mango, Cherimoya, Pecan, Feijoa, Date Palm and Cactus pear, for commercial cultivation, was carried out.
  • Propagative material of Avocado, Kiwi and Banana was produced and distributed to farmers in the framework of development programs of the Ministry of Agriculture.
  • Improvement in the propagation technique of Kiwi (rooting cuttings, tissue culture), of Avocado (grafting, tissue culture), of Cherimoya (rooting cuttings, tissue culture) and of Banana - Pistachio - Olive - Rubus (by tissue culture) was achieved.
  • Modern cultivation techniques on plantation systems, on plant size and structure, on pruning and on fruit thinning for the fruit quality improvement of the plants Kiwi, Avocado, Banana, Mango, Cherimoya, Pecan, Feijoa, Loquat and Cactus pear were applied and evaluated.
  • Cultivation techniques for the production of out of season Mango fruits by intensive cultivation under greenhouse were developed.
  • The harvesting indexes for Avocado and Kiwi fruits as they were affected by the crop location were determined

citrus3 citrus2 

Research Projects
  • Fruits of the late lemon variety 'Vakalou' in comparison to the 'Eureka Frost'.
  • "Productive Development of Drought Tolerant Species for Arid and Semi-arid Zones". FONDEF. - Ε.Ε.
  • "Mediterranean Selected Fruits Inter-country Network". (MESFIN). - E.E.
  • "Quality enhancement of plant production through tissue culture". COST843 - .Ε.Ε.
  • "Viruses: Study of molecular interactions with the host and epidemiology of Exocortis of citrus fruits" GSRT - PENED 99.
  • "Hydrogenases and environmental biotechnology". COST 818 – E.U.
  • "Comparative study of varieties and species of citrus fruits in the region Crete".
  •  Αντιμετώπιση της ίωσης Tristeza των εσπεριδοειδών στην Ελλάδα και Κύπρο. Διμερής συνεργασία Ελλάδας – Κύπρου
  • "The aphids species of citrus plants in the region of Chania and their role to the virus disease Tristeza" Ministry of Agriculture
  • "New citrus rootstocks: Micropropagation, molecular-genetic identification and their evaluation in biotic and abiotic factors" 'ΕSPERYPO' (ΑΕΙΦΟ/0506/02) in the Bilateral Cooperation of Greece-Cyprus.
  • "Safe and High Quality Chains and Networks for the Citrus Industry Between Mediterranean Partner Countries and Europe", (EuroMed CitrusNet) in FP6-2005-Food-4 C of ΕU.
  • COST FA1103 (2011-2015) με συμμετοχή 25 Ευρωπαϊκών χωρών  με θέμα «Endophytes in Biotechnology and Agriculture»
  • «Συμβολή των μυκορριζών στην αειφορικότητα οριακών μεσογειακών συστημάτων – ανάπτυξη μυκορριζικών εμβολίων» (2012-2015) Η πρόταση εκκρίθηκε στα πλαίσια της πράξης 'ΘΑΛΗΣ' 'Ενίσχυση της Διεπιστημονικής ή και Διαϊδρυματικής έρευνας και καινοτομίας' του Υπ. Παιδείας, δια Βίου Μάθησης και Καινοτομίας.
  • Lionakis, S. M. and Loxou, B. K. 1996. Behaviour of some Mango cultivars in the greenhouse, under net and outdoors in the area of Chania Crete. Acta Horiculturae, 455, 401-406.
  • Lionakis, S. M., and Schwabe, W. W. 1984. Some effects of daylength Temperature and exogenous growth regulator application on the growth of Actinidia chinensis Planch. Annals of Botany 54, 485-501.
  • Lionakis, S. M., and Schwabe, W. W. 1985. Some effects of mineral nutrient deficiences on the growth of Actinidia chinensis Planch. Journal of Hort. Science 60 (3), 411-422.
  • Vekiari, S. and Lionakis, S. M. 1988. Fruit quality characteristics of some Avocado cultivars in Greece. Proceedings of 2nd International meeting on Mediterranean tree crops. Chania 2-4 November 1988
  • Souad, B., Lionakis, S. M. and Gerasopoulos, D. 1998. Effects of the explant type on proliferation and rooting of kiwi in vitro. Adv. Hort. Sci., 12(3), 123-126.
  • Protopapadakis, , E. and Papanikolaou, X. 1998. Characterization of Citrus aurantium and Citrus taiwanica rootstocks by isozymes and essential oil analysis. Jour. Hort. Sci. Biot 73(1) 81-85.
  • Protopapadakis, E. and Papanikolaou, X. 1998. A study on Glutamate - Oxaloacetate Transaminase isozymes of Citron cultivars. Genetic Resources and Crop Evolution 45: 561-564.
  • Protopapadakis, E., Voulgaropoulous, A. And Sofoniou, M. 1998. Roostocks affect leaf and fruit mineral concentrations of washington navel orange. Fruits 53 (3):167-173.
  • Protopapadakis, E. and Papanikolaou X. 1999. Use of four isozymatic systems in lemon and lemon -like citrus cultivars to detect their genetic diversity Jour. Hort. Sci. Biot 73(6) :26-29.
  • Protopapadakis, E., Papadakis, J. and Kasapakis, J. 2004. Collapse of "Encore" Mandarin due to Nutritional Deficiencies. Proc. Int. Soc. Citriculture.
  • Papadakis, J., Protopapadakis, E., Therios, J. 2004. Nutritional status, yield and fruit quality of "Encore" mandarin in two sites. Journal of Plant Nutrition, vol. 27 (9), 1505-1515.
  • Papadakis, J., Protopapadakis, E., Therios, J. 2008. Yield and fruit quality of two late-maturing Valencia orange tree varieties as affected by harvest date. FRUITS 63 (6): 327-334.
  • Kavroulakis, N., Ntougias, S., Zervakis, G.I., Ehaliotis C., Haralampidis K., Papadopoulou, K.K. 2007.  Role of ethylene in the protection of tomato plants against fungal pathogens conferred by an endophytic Fusarium solani strain. Journal of Experimental Botany 58, 3853-3864.
  • Ntougias, S., Papadopoulou.K.K, Zervakis, G.I., Kavroulakis, N. and Ehaliotis, C. 2008. Suppression of soil-borne pathogens of tomato by composts deriving from agro-industrial wastes abundant in Mediterranean regions. Biology and Fertility of Soils 44, 1081-1090.
  • Kavroulakis, N., Ntougias, S., Besi, M., Katsou, P., Damaskinou, A., Ehaliotis, C., Zervakis, I. G., Papadopoulou K.K. 2010. Antagonistic bacteria of composted agro-industrial residues exhibit antibiosis against soil-borne fungal plant pathogens and protection of tomato plants from Fusarium oxysporum f.sp. radicis-lycopersici. Plant and Soil 333, 233-247.
  • Ntougias, S., Kavroulakis, N., Zervakis, G.I., Papadopoulou.K.K. and Ehaliotis, C. 2010. Characterisation of Cultivated Fungi Isolated from Grape Marc Wastes through the Use of Amplified rDNA Restriction Analysis and Sequencing. The Journal of Microbiology 48, 297-306.
  • Kavroulakis, N. and Ntougias, S. 2011. Bacterial and beta-proteobacterial diversity in Olea europaea var. mastoidis- and O. europaea var. koroneiki-generated olive mill wastewaters: influence of cultivation and harvesting practice on bacterial community structure. World Journal of Microbiology and Biotechnology 27, 57–66.
  • Delis, C., Krokida, A., Georgiou, S., Peña-Rodríguez, L.M., Kavroulakis, N., Ioannou, E., Roussis, V., Osbourn, A.E. and Papadopoulou K.K. 2011. Role of lupeol synthase in Lotus japonicus nodule formation. New Phytologist  189, 335-346.

 Dr. Kavroulakis Nektarios 

Agronomist, Head of the Laboratoty

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Tel: 28210-83430



Mikalef Louiza

Agricultural Techologist

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Tel: 28210-83433

 Salloustrou Sofia


Tel: 28210-83433

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